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60 × oil objective on a deltavision deconvolution fluorescence microscope  (Applied Precision Inc)

 
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    Applied Precision Inc 60 × oil objective on a deltavision deconvolution fluorescence microscope
    Nocodazole treatment leads to a transient decrease in linear movement of intracellular capsids. CMMT cells were co-transfected with pSARM–GagGFP–M100A and pTML-Env–mCherry and visualized in real time with a <t>Deltavision</t> <t>deconvolution</t> <t>microscope.</t> Live imaging of untreated cells and those treated with nocodazole for 2 h and 4 h was performed. Videos were recorded over a 2 min time period, with each video having an identical frame rate of five frames per second.(A) Illustration of tracks for Gag–GFP (green), Env–mCherry (red) and co-localized Gag–Env particles (yellow) in untreated and nocodazole-treated cells. Tracks shown are for particles moving towards or near the plasma membrane, with arrow heads indicating direction of movement. Data shown is representative of at least five independent imaging experiments. Bars=15 μm. See accompanying Movies S2–S4 for real time visualization of particle tracking. (B) Maximum velocity measurements of Gag–GFP particles (n ≥ 15), Env– mCherry particles (n ≥ 10) and co-localized Gag–Env particles (n ≥ 23) in untreated cells and nocodazole-treated cells after 2 h and 4 h of drug treatment. A minimum of 5 cells was analyzed at each time point. Bars indicate median values. Statistical significance is denoted by p value or ns (not significant).
    60 × Oil Objective On A Deltavision Deconvolution Fluorescence Microscope, supplied by Applied Precision Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/60+%C3%97+oil+objective+on+a+deltavision+deconvolution+fluorescence+microscope/60+%C3%97+oil+objective+on+a+deltavision+deconvolution+fluorescence+microscope/pmc04219502-345-11-15
    Average 90 stars, based on 1 article reviews
    60 × oil objective on a deltavision deconvolution fluorescence microscope - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Direct evidence for intracellular anterograde co-transport of M-PMV Gag and Env on microtubules"

    Article Title: Direct evidence for intracellular anterograde co-transport of M-PMV Gag and Env on microtubules

    Journal: Virology

    doi: 10.1016/j.virol.2013.11.006

    Nocodazole treatment leads to a transient decrease in linear movement of intracellular capsids. CMMT cells were co-transfected with pSARM–GagGFP–M100A and pTML-Env–mCherry and visualized in real time with a Deltavision deconvolution microscope. Live imaging of untreated cells and those treated with nocodazole for 2 h and 4 h was performed. Videos were recorded over a 2 min time period, with each video having an identical frame rate of five frames per second.(A) Illustration of tracks for Gag–GFP (green), Env–mCherry (red) and co-localized Gag–Env particles (yellow) in untreated and nocodazole-treated cells. Tracks shown are for particles moving towards or near the plasma membrane, with arrow heads indicating direction of movement. Data shown is representative of at least five independent imaging experiments. Bars=15 μm. See accompanying Movies S2–S4 for real time visualization of particle tracking. (B) Maximum velocity measurements of Gag–GFP particles (n ≥ 15), Env– mCherry particles (n ≥ 10) and co-localized Gag–Env particles (n ≥ 23) in untreated cells and nocodazole-treated cells after 2 h and 4 h of drug treatment. A minimum of 5 cells was analyzed at each time point. Bars indicate median values. Statistical significance is denoted by p value or ns (not significant).
    Figure Legend Snippet: Nocodazole treatment leads to a transient decrease in linear movement of intracellular capsids. CMMT cells were co-transfected with pSARM–GagGFP–M100A and pTML-Env–mCherry and visualized in real time with a Deltavision deconvolution microscope. Live imaging of untreated cells and those treated with nocodazole for 2 h and 4 h was performed. Videos were recorded over a 2 min time period, with each video having an identical frame rate of five frames per second.(A) Illustration of tracks for Gag–GFP (green), Env–mCherry (red) and co-localized Gag–Env particles (yellow) in untreated and nocodazole-treated cells. Tracks shown are for particles moving towards or near the plasma membrane, with arrow heads indicating direction of movement. Data shown is representative of at least five independent imaging experiments. Bars=15 μm. See accompanying Movies S2–S4 for real time visualization of particle tracking. (B) Maximum velocity measurements of Gag–GFP particles (n ≥ 15), Env– mCherry particles (n ≥ 10) and co-localized Gag–Env particles (n ≥ 23) in untreated cells and nocodazole-treated cells after 2 h and 4 h of drug treatment. A minimum of 5 cells was analyzed at each time point. Bars indicate median values. Statistical significance is denoted by p value or ns (not significant).

    Techniques Used: Transfection, Microscopy, Imaging, Clinical Proteomics, Membrane

    Related Articles

    Fluorescence:

    Article Title: Direct evidence for intracellular anterograde co-transport of M-PMV Gag and Env on microtubules
    Article Snippet: After washing twice with 1 × PBS, cover-slips were mounted in ProLong antifade reagent (Invitrogen) containing DAPI for nuclei staining. .. Cells were visualized using a 60 × oil objective on a Deltavision deconvolution fluorescence microscope (Applied Precision Inc., Issaquah, WA). .. Images were acquired and processed using Deltavision SoftWoRx software (Applied Precision Inc., Issaquah, WA).

    Microscopy:

    Article Title: Direct evidence for intracellular anterograde co-transport of M-PMV Gag and Env on microtubules
    Article Snippet: After washing twice with 1 × PBS, cover-slips were mounted in ProLong antifade reagent (Invitrogen) containing DAPI for nuclei staining. .. Cells were visualized using a 60 × oil objective on a Deltavision deconvolution fluorescence microscope (Applied Precision Inc., Issaquah, WA). .. Images were acquired and processed using Deltavision SoftWoRx software (Applied Precision Inc., Issaquah, WA).



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    60 × oil objective on a deltavision deconvolution fluorescence microscope  (Applied Precision Inc)
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    Applied Precision Inc 60 × oil objective on a deltavision deconvolution fluorescence microscope
    Nocodazole treatment leads to a transient decrease in linear movement of intracellular capsids. CMMT cells were co-transfected with pSARM–GagGFP–M100A and pTML-Env–mCherry and visualized in real time with a <t>Deltavision</t> <t>deconvolution</t> <t>microscope.</t> Live imaging of untreated cells and those treated with nocodazole for 2 h and 4 h was performed. Videos were recorded over a 2 min time period, with each video having an identical frame rate of five frames per second.(A) Illustration of tracks for Gag–GFP (green), Env–mCherry (red) and co-localized Gag–Env particles (yellow) in untreated and nocodazole-treated cells. Tracks shown are for particles moving towards or near the plasma membrane, with arrow heads indicating direction of movement. Data shown is representative of at least five independent imaging experiments. Bars=15 μm. See accompanying Movies S2–S4 for real time visualization of particle tracking. (B) Maximum velocity measurements of Gag–GFP particles (n ≥ 15), Env– mCherry particles (n ≥ 10) and co-localized Gag–Env particles (n ≥ 23) in untreated cells and nocodazole-treated cells after 2 h and 4 h of drug treatment. A minimum of 5 cells was analyzed at each time point. Bars indicate median values. Statistical significance is denoted by p value or ns (not significant).
    60 × Oil Objective On A Deltavision Deconvolution Fluorescence Microscope, supplied by Applied Precision Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/60+%C3%97+oil+objective+on+a+deltavision+deconvolution+fluorescence+microscope/60+%C3%97+oil+objective+on+a+deltavision+deconvolution+fluorescence+microscope/pmc04219502-345-11-15
    Average 90 stars, based on 1 article reviews
    60 × oil objective on a deltavision deconvolution fluorescence microscope - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Nocodazole treatment leads to a transient decrease in linear movement of intracellular capsids. CMMT cells were co-transfected with pSARM–GagGFP–M100A and pTML-Env–mCherry and visualized in real time with a Deltavision deconvolution microscope. Live imaging of untreated cells and those treated with nocodazole for 2 h and 4 h was performed. Videos were recorded over a 2 min time period, with each video having an identical frame rate of five frames per second.(A) Illustration of tracks for Gag–GFP (green), Env–mCherry (red) and co-localized Gag–Env particles (yellow) in untreated and nocodazole-treated cells. Tracks shown are for particles moving towards or near the plasma membrane, with arrow heads indicating direction of movement. Data shown is representative of at least five independent imaging experiments. Bars=15 μm. See accompanying Movies S2–S4 for real time visualization of particle tracking. (B) Maximum velocity measurements of Gag–GFP particles (n ≥ 15), Env– mCherry particles (n ≥ 10) and co-localized Gag–Env particles (n ≥ 23) in untreated cells and nocodazole-treated cells after 2 h and 4 h of drug treatment. A minimum of 5 cells was analyzed at each time point. Bars indicate median values. Statistical significance is denoted by p value or ns (not significant).

    Journal: Virology

    Article Title: Direct evidence for intracellular anterograde co-transport of M-PMV Gag and Env on microtubules

    doi: 10.1016/j.virol.2013.11.006

    Figure Lengend Snippet: Nocodazole treatment leads to a transient decrease in linear movement of intracellular capsids. CMMT cells were co-transfected with pSARM–GagGFP–M100A and pTML-Env–mCherry and visualized in real time with a Deltavision deconvolution microscope. Live imaging of untreated cells and those treated with nocodazole for 2 h and 4 h was performed. Videos were recorded over a 2 min time period, with each video having an identical frame rate of five frames per second.(A) Illustration of tracks for Gag–GFP (green), Env–mCherry (red) and co-localized Gag–Env particles (yellow) in untreated and nocodazole-treated cells. Tracks shown are for particles moving towards or near the plasma membrane, with arrow heads indicating direction of movement. Data shown is representative of at least five independent imaging experiments. Bars=15 μm. See accompanying Movies S2–S4 for real time visualization of particle tracking. (B) Maximum velocity measurements of Gag–GFP particles (n ≥ 15), Env– mCherry particles (n ≥ 10) and co-localized Gag–Env particles (n ≥ 23) in untreated cells and nocodazole-treated cells after 2 h and 4 h of drug treatment. A minimum of 5 cells was analyzed at each time point. Bars indicate median values. Statistical significance is denoted by p value or ns (not significant).

    Article Snippet: Cells were visualized using a 60 × oil objective on a Deltavision deconvolution fluorescence microscope (Applied Precision Inc., Issaquah, WA).

    Techniques: Transfection, Microscopy, Imaging, Clinical Proteomics, Membrane